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KMID : 0364819910290010008
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Korean Journal of Microbiology
1991 Volume.29 No. 1 p.8 ~ p.15
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Construction of Interspecific Hybrid between Aspergillus spp. By Nuclear Transfer
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Rho, Hyung Seon/노형선
Lee, Jeong Ae/Rhee, Young Ha/Kim, Jin Mi/Chung, Jae Hoon/Maeng, Pil Jae/이정애/이영하/김진미/정재훈/맹필재
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Abstract
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1
Interspecific hybrids between the Aspergiius spp., A. awamori, A. usamii and A. oyzae, were obtained by nuclear transfer technique. Nuclei isolated from an auxotrophic mutant strain were transferred into the protoplasts of a recipient strain of different species. The frequency of interspecific hybrid formation by nuclear transfer was 2X10`-7X10-`. In contrast, no interspecific hybrid was isolated by protoplast fusion. Among the hybrids tested, 10 strains showed increased activity of some or all components of cellulases, xylanases and amylase up to more than two times. Isozyme patterns of the hybrids were analyzed by polyacrylamide gel electrophoresis and isoelectric focusing followed by activity staining, which showed that some of the hybrids have isozyme patterns unidentical to either of the two parents. By measuring the DNA contents and the sizes of the conidia, the karyotypes of the hybrids were estimated to be aneuploid near to haploid, diploid or triploid. It was concluded that the nuclear transfer technique is much more efficient in the formation of interspecific hybrids than protoplast fusion, and is very useful for the improvement of Aspergillus strains.
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